Lumbar Puncture:
This (Really) Is Spinal Tap

Gary E Cordingley, MD, PhD
While devised over a century ago, the medical test known as lumbar puncture is still the
gold-standard procedure for diagnosing a number of serious conditions affecting the
brain and spinal cord.
I couldn't resist the title's corny riff on the name of the rock band and their movie, but the kind of spinal tap
featured in this article was a spinal tap before Spinal Tap was Spinal Tap. (Does that make any sense?)

Known more formally as a lumbar puncture, this kind of spinal tap is a valuable medical test with an interesting
history. In 1891 Heinrich Quincke, of Kiel, Germany, introduced this procedure as we know it today. His original
intent was to help babies suffering from hydrocephalus (water on the brain) by draining away excess fluid, but
from the outset he was also interested in lumbar puncture's use as a diagnostic tool.

To understand the usefulness of this test and why you might someday need to have one, a little background is
helpful. The brain and spinal cord are wrapped in a membrane called the meninges.  Within the meninges, a
watery fluid called the cerebrospinal fluid (CSF) bathes the inside and outside of the brain and the outside of
the spinal cord. Within the brain's fluid chambers (ventricles), the body perpetually manufactures new CSF from
constituents of the bloodstream. Once the CSF has percolated through openings to get outside the brain, it is
reabsorbed and recycled into the bloodstream. The entire volume of CSF—about 150 milliliters or five ounces—
is made and reabsorbed several times per day.

Dr. Quincke understood that analyzing the CSF's makeup could be useful in diagnosing infections and other
diseases affecting the central nervous system (brain plus spinal cord). Measuring the CSF's protein and
glucose (sugar) content along with inspecting a sample of CSF under a microscope to count red and white
blood-corpuscles soon became standard practices.

The premier use of lumbar puncture in both Quincke's time and ours has been to diagnose meningitis. The
suffix "-itis" signifies inflammation, so meningitis means inflammation of the meninges. Most, but not all,
instances of meningitis are due to infections, but the kinds of infection seen have evolved over the years. In
Quincke's lifetime tuberculosis and syphilis germs were common causes of meningitis, but presently, in
developed countries these are uncommon. Nowadays, the usual causes of meningitis are other bacteria,
viruses or even funguses. In cases of suspected infection, CSF protein, glucose and blood-corpuscle
measurements are supplemented by other tests on the fluid that can track down the specific, infecting

Another important use of lumbar puncture is to diagnose subarachnoid hemorrhage, an abrupt, devastating,
and potentially lethal bleed into the CSF space caused by rupture of an aneurysm or other abnormal blood
vessel. In suspected cases—classically presenting with "the worst headache of my life"—a computed
tomographic (CT) scan is usually performed first. While very sensitive in detecting subarachnoid hemorrhages,
CT scans can still miss cases. So if the doctor is still suspicious that a bleed occurred, the next step is to do a
lumbar puncture which is 100% sensitive in detecting this condition. That is, it never misses.

Lumbar puncture with CSF analysis can also help in the diagnosis of multiple sclerosis, a disease in which the
patient's own immune system attacks the central nervous system. In this condition the immune reaction
produces abnormal proteins that can be detected and measured in the CSF.

How is the test performed? Well, the first step, of course, is the informed consent process in which your doctor
explains the risks and benefits of the test and you sign a permission form. In this author's opinion, lumbar
puncture is the most benign test for which written permission is traditionally required and is less risky than some
other procedures—like drawing blood from a high-pressure artery—for which written permission is traditionally

The next step is to lie on your side on a bed or procedure table with your knees tucked up to your chest. The
skin of your lower back is painted with an iodine-based solution to produce a sterile field. If you have an allergy
to iodine, an alcohol-based solution is substituted. The surrounding area is then covered with sterile paper or
cloth. The skin and the tissue beneath the skin are then numbed with local anesthetic, and then everything is
ready to insert the spinal needle.

The reason the lower back (lumbar spine) is chosen is because here the sac of meninges can be entered
without risk of poking a hole in the spinal cord. This is because the spinal cord ends several inches higher
within the spinal canal. The composition of the CSF is nearly the same throughout its system. Thus, CSF from
the lumbar region is as good for diagnosis as from anywhere else, yet safer to obtain.

Once the spinal needle enters the lumbar sac of fluid, correct positioning of the needle is confirmed by the
emergence of clear, colorless drops of fluid from the back of the needle. (When a similar procedure is
performed for the purpose of epidural anesthesia, the tip of the needle stops just short of entering the
meninges, and the drug is infused outside the sac.) A thin plastic tube is then attached to the back of the
needle so the CSF's pressure can be measured. Subsequently, CSF is allowed to drip into each of several
sealable test-tubes suitable for sending to the laboratory.

Once adequate fluid has been obtained, the needle is withdrawn and the small puncture site in the skin is
covered with an adhesive bandage. Typically, there are no more than a few drops of blood-loss from this test.

How about risks? Fortunately, they are minimal. As with any other test in which a needle is inserted somewhere
that Mother Nature never intended, bleeding is a possibility. Luckily, there are no major blood-vessels in the
vicinity, so even an off-course needle is unlikely to cause trouble. Theoretically, a needle-insertion could also
bring germs into the body and cause infection, but this almost never occurs because the needle is sterile and
because the lumbar region had been surgically prepped.

About one-in-five patients experiences a headache from the procedure. When a spinal-tap headache occurs, it
always has the following characteristics: it is present while the patient is sitting or standing, and is promptly
relieved by lying down. Spinal-tap headaches are due to persistent leaking of CSF through the hole that the
needle made in the meninges. (The leaking occurs within the spinal column and doesn't leave the body.) Until
the hole seals up again and the full volume of CSF is restored, the CSF cannot provide its usual cushioning
effect with changes in head position, and a headache ensues. In such cases the patient remains horizontal until
the leak has sealed over.

Reviewing a list of potential complications can have a discouraging effect on people who need a test. But it is
reassuring to know that millions of people have had Dr. Quincke's test since he devised it over a century ago. If
the test caused unforeseen problems, they should have turned up by now.

(C) 2005 by Gary Cordingley